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anti cd4 alexa fluor 647  (Bio-Rad)


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    Bio-Rad anti cd4 alexa fluor 647
    Anti Cd4 Alexa Fluor 647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bovine+cd4/pm41271900-321-17-21?v=Bio-Rad
    Average 94 stars, based on 137 article reviews
    anti cd4 alexa fluor 647 - by Bioz Stars, 2026-06
    94/100 stars

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    Proliferation of CD4 + T cells from RB51 vaccinated dairy-beef heifers in response to in vitro RB51 and PPDb stimulation. Mean numbers (gray bars) of CD4 + T cells proliferating in response to media only (no stimulation, white circles, n = 8), γ-irradiated RB51 (red circles, n = 8), and PPDb (blue circles, n = 8). *, RB51 or PPDb stimulated CD4 + T cells compared to unstimulated CD4 + T cells at respective time points. #, PPDb stimulated CD4 + T cells compared to RB51 stimulated CD4 + T cells at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. *, #p ≤ 0.05; **p ≤ 0.01; ***, ###p ≤ 0.001 .

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Proliferation of CD4 + T cells from RB51 vaccinated dairy-beef heifers in response to in vitro RB51 and PPDb stimulation. Mean numbers (gray bars) of CD4 + T cells proliferating in response to media only (no stimulation, white circles, n = 8), γ-irradiated RB51 (red circles, n = 8), and PPDb (blue circles, n = 8). *, RB51 or PPDb stimulated CD4 + T cells compared to unstimulated CD4 + T cells at respective time points. #, PPDb stimulated CD4 + T cells compared to RB51 stimulated CD4 + T cells at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. *, #p ≤ 0.05; **p ≤ 0.01; ***, ###p ≤ 0.001 .

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: In Vitro, Irradiation

    Proliferative CD4 + T cell responses in combo vaccinated dairy-beef heifers compared to RB51 or BCG vaccinates. (A) Mean numbers of RB51-specific CD4 + T cells (gray bars) in combo vaccinated heifers (black circles, n = 8) compared to RB51 vaccinated heifers (red circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both RB51 and combo vaccinated heifers was used as a control. (B) Mean numbers (gray bars) of BCG-specific CD4 + T cells in combo vaccinated heifers (black circles, n = 8) compared to BCG vaccinated heifers (blue circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both BCG and combo vaccinated heifers was used as a control. *, proliferating CD4 + T cells from combo vaccinated heifers compared to single vaccinated heifers at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 .

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Proliferative CD4 + T cell responses in combo vaccinated dairy-beef heifers compared to RB51 or BCG vaccinates. (A) Mean numbers of RB51-specific CD4 + T cells (gray bars) in combo vaccinated heifers (black circles, n = 8) compared to RB51 vaccinated heifers (red circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both RB51 and combo vaccinated heifers was used as a control. (B) Mean numbers (gray bars) of BCG-specific CD4 + T cells in combo vaccinated heifers (black circles, n = 8) compared to BCG vaccinated heifers (blue circles, n = 8). Proliferation in unstimulated (white circles, n = 16) CD4 + T cells from both BCG and combo vaccinated heifers was used as a control. *, proliferating CD4 + T cells from combo vaccinated heifers compared to single vaccinated heifers at respective time points. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 .

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: Control

    Th1 functional potential of vaccine-specific CD4 + T cells in dairy-beef heifers. (A) Quadrant gate defines functional subsets of CD4 + T cells: Q1, proliferating and IFN-γ + ; Q2, IFN-γ + only; Q3, unresponsive; Q4, proliferating only. (B, C) Bar height represents total average number of responding CD4 + T cells; broken down into IFN-γ + only (white bars), proliferating only (gray bars), and proliferating and IFN-γ + (black bars) subsets. (B) Mean number of RB51-specific CD4 + T cells exhibiting Th1 functionality in RB51 vaccinated (n = 8) and combo vaccinated (n = 8) heifers. (C) Mean number of BCG-specific CD4 + T cells exhibiting Th1 functional potential in BCG vaccinated (n = 8) and combo vaccinated (n = 8) heifers.

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Th1 functional potential of vaccine-specific CD4 + T cells in dairy-beef heifers. (A) Quadrant gate defines functional subsets of CD4 + T cells: Q1, proliferating and IFN-γ + ; Q2, IFN-γ + only; Q3, unresponsive; Q4, proliferating only. (B, C) Bar height represents total average number of responding CD4 + T cells; broken down into IFN-γ + only (white bars), proliferating only (gray bars), and proliferating and IFN-γ + (black bars) subsets. (B) Mean number of RB51-specific CD4 + T cells exhibiting Th1 functionality in RB51 vaccinated (n = 8) and combo vaccinated (n = 8) heifers. (C) Mean number of BCG-specific CD4 + T cells exhibiting Th1 functional potential in BCG vaccinated (n = 8) and combo vaccinated (n = 8) heifers.

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: Functional Assay

    Proliferation of CD4 + T cells in response to in vitro PPDb stimulation in BCG and combo vaccinated Holstein steers. The average number of CD4 + T cells (gray bars) proliferating in response to PPDb stimulation from unvaccinated (white circles, n = 8), BCG vaccinated (blue circles, n = 7), and combo vaccinated (black circles, n = 8) Holstein steers. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance .

    Journal: Frontiers in Immunology

    Article Title: Effect of co-vaccination of cattle with RB51 and BCG on vaccine-specific CD4 + T cell responses

    doi: 10.3389/fimmu.2025.1664398

    Figure Lengend Snippet: Proliferation of CD4 + T cells in response to in vitro PPDb stimulation in BCG and combo vaccinated Holstein steers. The average number of CD4 + T cells (gray bars) proliferating in response to PPDb stimulation from unvaccinated (white circles, n = 8), BCG vaccinated (blue circles, n = 7), and combo vaccinated (black circles, n = 8) Holstein steers. AR1, circles represent individual animal cell counts and gray bars represent means ± SEM. NS, no significance .

    Article Snippet: Cells were then incubated with FITC labeled anti-bovine CD4 (clone CC8, Cat. No. MCA1653F, BioRad, Hercules, CA) antibody for 15 min at RT.

    Techniques: In Vitro